CBSE 2024-25 Competency-Based Questions With Answers: Assessment is a part of learning that leads to the foundation of knowledge gained by the students throughout the course. Competency-based questions are designed to evaluate the candidate’s knowledge of a particular subject by assessing their skills, abilities and behaviours.
The Central Board Of Secondary Education has released the 2024-25 competency-based questions for classes 12 and if the students want to take a look at it, then we will be providing the questions with answers and that too chapter-wise.
Here, we provide the CBSE Class 12 Biology Chapter 2 Biotechnology And Its Applications competency-based questions for the 2025 examination. The free PDF for Biology Volume 2 has also been provided so that the students can download the link to it easily.
CBSE Class 12 Biology Chapter 2 Biotechnology And Its Applications Competency-Based Questions
1. There are two statements given below marked as Assertion (A) and Reason (R). Read the statements and choose the correct option.
Assertion: RNA interference (RNAi) is used to protect plants against nematode infestation.
Reason: The RNAi method involves silencing of a specific mRNA by a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
A. A is true but R is false.
B. A is false but R is true.
C. Both A and R are true and R is the correct explanation of A.
D. Both A and R are true and R is not the correct explanation of A.
2. Given below are involved in the gene therapy of a patient with adenosine deaminase (ADA) deficiency.
P) Lymphocytes are grown in a culture outside the human body.
Q) Lymphocytes are altered with a functional ADA cDNA.
R) Lymphocytes are collected from the blood of the patients.
S) The genetically modified lymphocytes are infused back into the patient.
3. Which of the following shows the correct order in which these steps must occur?
A. Q --> P --> R --> S
B. R --> S --> Q --> P
C. R --> P --> Q --> S
D. S --> R --> P --> Q
4. Given below is an image of the process involved in recombinant DNA technology.
LacZ is a gene that codes of β-galactosidase. When plated on an appropriate medium, cells that produce β-galactosidase form blue colonies whereas those that do not produce it form white colonies. Which of the following chemicals will be involved in a step that would come before step 1?
lysozyme, ethanol, ribonuclease, cellulase, protease
A. only lysozyme and ethanol
B. only cellulase, lysozyme and ethanol
C. only lysozyme, ribonuclease, protease, and ethanol
D. all - lysozyme, ethanol, ribonuclease, cellulase, protease
5. Which of the following will help one detect cells containing the recombinant DNA?
A
B
C
D
6. At which step will DNA ligase be required?
A. step 2
B. step 3
C. step 4
D. (DNA ligase is not required in this process)
7. What would be the colour of the colonies formed by variant P?
A. only blue colonies
B. only white colonies
C. a mix of blue and white colonies
D. (They will not form any colonies.)
8. Polymerase chain reaction (PCR) mimics which of the following natural processes?
A. ligation
B. translation
C. replication
D. transformation
9. Which of the following correctly states the purpose of steam in a bio-processor?
A. helps organisms grow faster
B. adds to the water content
C. maintains sterility
D. (Steam is not required in a bio-processing unit.)
10. Similar to sexual reproduction, rDNA technology contributes to
P) genetic mutation
Q) faster replication
R) genetic variation
S) DNA ligation
A. only R
B. only P and R
C. only P, Q and R
D. all - P, Q, R and S
11. In which of the following natural processes is recombinant DNA made?
A. prophase in mitosis
B. prophase 1 in meiosis
C. prophase 2 in meiosis
D. (Recombinant DNA cannot be made naturally).
12. Two statements are given below - one labelled Assertion (A) and the other labelled Reason (R).
Assertion (A): Sexual reproduction is similar to rDNA technology because it results in variations.
Reason (R): Variation is impossible through asexual reproduction.
Which of the following is correct?
A. Both A and R are true, and R is the correct explanation for A.
B. Both A and R are true, but R is not the correct explanation for A.
C. A is true, but R is false.
D. A is false, but R is true
13. Two statements are given - one labelled Assertion (A) and the other labelled Reason (R).
Assertion (A): Gene therapy is a method of treating a disorder but cannot cure it.
Reason (R): In gene therapy, cells are drawn from a patient and the functional gene is introduced into these cells and transferred back to the patient.
Which of the following is correct?
A. Both A and R are true, and R is the correct explanation for A.
B. Both A and R are true, but R is not the correct explanation for A.
C. A is true, but R is false.
D. A is false, but R is true.
14. Given below are two statements about polymerase chain reactions.
P) It mimics DNA replication that happens in a cell.
Q) It cannot be used to amplify RNA molecules.
Which of these is/are TRUE?
A. only P
B. only Q
C. both P and Q
D. neither P nor Q
15. X is the first restriction enzyme isolated from strain H of Bacillus amyloliquefaciens. This enzyme cuts between two guanine bases of the following palindromic sequence:
5’-G G A T C C-3’
3’-C C T A G G-5’
(a) Name the restriction enzyme X. Explain how you arrived at this name.
(b) Draw the DNA fragments produced due to the action of enzyme X on the palindromic sequence shown above.
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To get the other questions, we provide the free link below to download the question-answer PDF.
CHECK: CBSE Class 12 Biology Chapter 2 Biotechnology And Its Applications Competency-Based Questions
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